Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Why was sonication unsuccessful for 1 of 6 samples?

I have sonicated six HeLa-cell samples in RIPA lysis buffer (prior inc. on ice, 15 min). For one of these samples, the sonication was unsuccessful. My question is what factors should be taken into...

17 December 2020 8,531 2 View

Maskning for polysterene substrate?

We want to deposite a fine pattern ( 100-300 nm thick and 10 microns wide pattern) of Pt as temperature sensors on a polysterene substrate with a glass temperature of 95 degrees C. How would...

12 November 2020 9,761 3 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

Barriers and Factors with wound are discharge planning, how to narrow down this topic?

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down. Any ideas on wounds that need more...

29 May 2020 8,870 1 View

Why are some plasmids (such as pKD46) temperature sensitive? What happens at higher temperatures to cause plasmid loss?

I am trying to figure out how temperature sensitive plasmids are made/isolated and what the biochemical/cellular mechanism is that causes them to be lost/lose function at higher temperatures.

20 March 2020 6,815 3 View

Universal 16S bacterial primer for feces DNA for qPCR?

I already tested three different sequences and had problems with dimers and melt curve (low specificity). Any reliable primers? I'm studying rat microbiota with qPCR.

12 March 2020 7,658 2 View

In 6-line Ferrihydrite synthesis is dialysis necessary or can the salt be removed with washing?

I don't have dialysis tubing and am wondering if washing is sufficient.

09 March 2020 5,484 3 View

Clostridium spp. primer qPCR sequence?

19 January 2020 7,194 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

What is the best technique for combining images?

Hi, Sorry I don't have much experience in image processing. In my research, I need to combine 2 grayscale images(like attached images) into one! I came across the image fusion process. In the...

16 February 2021 7,317 7 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View