In a rat spinal fusion. model, can I use the same sample for manual palpation and histology?

Amanda dos Santos Cavalcanti @Amanda-Cavalcanti

26 September 2023 0 8K Report

I am concerned that the manual palpation may "harm" the fusion mass

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Amanda dos Santos Cavalcanti's questions See All

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Problem Justifcation of below research topic?

Analyzing the effectiveness of apply the Extended Producer Responsibility (EPR) concept for reducing pre-consumer apparel waste within a Star Fashion Pvt Ltd apparel company.

30 June 2024 3,764 0 View

Anyone familiar with the Bruker in-vivo F-PRO Photodocumentator?

Hello! I'm a PhD student and we need urgent technical help for the "Multimodal Pro Light Source 400W" equipment. We are having image acquisition problems in our laboratory. When we activate...

16 May 2024 9,545 0 View

Is there any adipocyte size for it to be considered unilocular?

I am evaluating histological slides (H&E) of brown adipose tissue from rats. And counting the number of unilocular adipocytes to evaluate the whitening process. How to identify? Would it...

13 May 2024 7,876 0 View

How to count brown/brite adipocites on microscope?

How to count multilocular adipocytes on a slide with a white adipose tissue sample stained with H&E? I had done the count on the entire slide, but the articles put it in % of cells or...

10 April 2024 3,997 2 View

Critically discuss complexity theory and the notion of resilience as they relate to public policy?

Explain the concept of complexity theory Discuss the link between resilience and complexity theory Explain how these relate to public policy illustrate your discussion with appropriate example

05 April 2024 8,886 1 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What evaluate to choose the best docking program for my protein and ligands?

I performed virtual screening of some molecules in Auto Dock Vina and GOLD and obtained different results for the best ligand. I'm not sure if I did something wrong, or the docking program is not...

07 March 2024 3,680 3 View

How to separate a misture of coordenation coumpounds that are water-soluble?

I did a synthesis of a carbonyl ruthenium complex coordinated with the 1H-imidazole-1-ylacetic acid, but I'm having problems in the purification process. All the mixture is only slightly soluble...

03 March 2024 2,041 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View