Hi there,
we are focused on impression cytology of conjunctiva. We would like to isolate the cells from imprints on strips of Millipore filter membranes. We´ve tried mechanical isolation/detachement into the DMEM with microcentrifuge tube shaker and then vortexing. However the yield and cell condition are not good. Simply, the most of the cells are damaged. Does anyone have some experience with the cell isolation from filter membranes? Any hint can be helpful. Thanks a lot!
Hello. We do not routinely take this exam. You've already seen this study. Maybe the article can help you.
Vet Ophthalmol. 2005 Nov-Dec; 8 (6): 401-5.
Conjunctival impression cytology in dogs. Bolzan AA.
In which species will you do the cytology ?
Good luck!
in 6s
Anyone who can host me in their lab to learn ICP measurement protocol? - ResearchGate. Available from: https://www.researchgate.net/post/Anyone_who_can_host_me_in_their_lab_to_learn_ICP_measurement_protocol#58c2bd40cbd5c278170e96c3 [accessed Mar 10, 2017].
In the publication attached, they use laser microdissection (MMI CellCut) to isolate cells from ISET filters. I hope this helps.
Article Technical Insights into Highly Sensitive Isolation and Molec...
- Badges
- Science method
- Similar topics
- Biological Science
- General Biochemistry
- Biochemical Techniques
- Cytology