Impact assessment paper of local food markets?

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Why battery always retain the same value of power?

Dear all, I have been testing a model that is in the Matlab library (https://es.mathworks.com/help/physmod/sps/ug/simplified-model-of-a-small-scale-micro-grid.html), this is named "power...

10 November 2020 2,117 3 View

Do I need to transform dependent variable if I am correcting for outliers?

Univariate normality test tells me I only have 2 values among my predictor variables that are >3 SD. Multivariate normality test tells me a have a few outliers. I also plotted residuals using...

27 October 2020 9,579 5 View

Would appreciate who can help me explain the results of the panel data analysis using STATA - Robust?

Some querries. A robust panel data analysis was done by my statistician in my study covering two periods: Panel A : 2008-2018 16 Oil companies and Panel B: 1988-2018 - Three Major Oil companies...

08 October 2020 940 3 View

Does grand mean centering affect the interpretation of unstandardized beta coefficients in any way?

Assuming I don't standardize beta coefficients (this was recommended for dyadic analysis), does anyone know if grand mean centering my predictors will help me better interpret beta coefficients?

16 September 2020 7,154 2 View

Need help understanding the value of grand mean centering in dyadic analysis?

Let's say my predictor values are: husbands' anxiety, wives' anxiety, husbands' satisfaction, and wives' satisfaction. I am conducting SEM. I am grand mean centering my predictors because it was...

15 September 2020 1,520 3 View

Has anynone successfully used NEB's TtAgo (Thermus Thermophilus Argonaute) for any type of application?

I have been trying to use NEB's TtAgo (https://www.neb.com/products/m0665-ttago) to linearize supercoiled plasmids, following NEB's recommendations, but I haven't had any success yet (19/19 failed...

09 September 2020 2,112 1 View

TCE Stock solution concentration for SDS-PAGE?

Greetings, I'm trying to standarize TCE free staining method to visualize protein bands over SDS-PAGE, it's never been used in our lab. I've seen many published papers using this method, and the...

17 August 2020 6,620 3 View

What are the limiting factors for drug development through electrospinning and scale-up of the process?

Making the assumption that both, drug and polymer are soluble in a suitable non-toxic solvent and that most of the solution's and process' parameters are well defined, what factors do you consider...

11 April 2020 5,041 5 View

Speed up the performance in DC-DC Power Converter?

Dear Ms, I have been thinking in how to Speed up the performance in DC-DC Power Converter?, because I have a systems which has 2 DC-DC converters, the first one is working in order to obtaing the...

30 January 2019 4,819 7 View

How to calculate the cost of building and maintain a grid-connected microgrid?

Dear Ms, I am seeking papers or official reports, in order to find, how do the plannifier determine and forecast the cost of implementing a microgrid?. Do you have information about it?, because I...

16 December 2018 153 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View