Dear all,
I am curious that if anyone knows whether surface markers such as F4/80, or CD206 are stable in paraffin sections?
The small intestine paraffin sections are about 3 years old, and I have tried multiple conditions but failed to stain F4/80. I also did the antigen retrieval for IF.
However, I have no issue when staining intracellular proteins, such as Ki-67 or Olfm4.
Therefore, I am curious if the surface markers decay faster?
Thank you in advance!
3 years old sections should not be a problem. In general Immunofluorescence works better on frozen sections and cells. For paraffin sections, try immunocytochemistry rather than immunofluorescence. That is my opinion.
Subhash C. Juneja Thank you!
Indeed 3 years old section is not a problem for surface marker F4/80.
Hi,
yes. I usually use DAB for counterstaining, definitely more stable in comparison with Immunofluorescence.
I can send you a protocol or suggest a working antibody, if needed.
Cheers,
Attila
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