hi everyone. I have done IF staining of psoriatic mice skin with UNC93B1. but I am unable to analyze. its my first time to do so. can someone help me.
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
So, I am working on a small protein (X) of Haloferax volcanii. It shows these results: 1. Mutant strain grows poorlycompared to wild type strain under low iron condition. 2. Growth of Mutant...
14 May 2024 9,037 4 View
Clarify a point for Multiepitope vaccine construction that 1) one should construct 3D structure of MEV with protein adjuvant sequence (A.A) or without it. 2)3D structure refinement, validation,...
06 April 2024 9,893 2 View
Hi everyone I want to introduce some iron stress to haloferax cells in the culture. Normally, i have been applying low iron stress where i just prepare media with low iron (Hv.Min minus 23 mg...
28 January 2024 5,294 0 View
Hello everyone I used WebFlaG for flanking genes homology search but I am not able to understand the result output completely. How to know what are these flanking genes? (digits for example). Does...
15 January 2024 8,126 0 View
In some cases i think blood group of a person changes . But not confirmed
02 January 2024 1,294 1 View
Hi everyone Its a small protein with 5.7kD weight. After doing weight adjustments considering acidic amino acids and also 3XFLAG tag, it should be 11.6 kD. But on 16% tricine gel, it appears...
14 August 2023 5,570 7 View
Hello all researchers: I have started working on a hypothetical protein. In silico (SWISS-prot, Hhpred, I-TASSER) analysis shows its homology to following proteins: 1. Hydrolase (peptidase) 2....
26 April 2023 6,021 2 View
I need following documents urgently because these document freely not available on ISO web site: ISO 22949-1:2021(MAIN) Molecular biomarker analysis — Methods of analysis for the detection and...
03 March 2023 5,612 0 View
I am working in a Molecular Biology Laboratory. I already have an UV trans Illuminator, but want to use Gel documentation system. because under transilluminator I experienced ambigious gel results...
24 February 2023 7,013 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View