I need to know their size,location,global or local,structure,employess. The companies are -The ordinary and Skin+ Me.
Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...
02 June 2024 5,489 2 View
My aim is to simulate cnt with aminoacids(histidine) in water solution. I`ve made two separate topology files(15 molecules of histidine with pdb2gmx command and cnt topology with x2top) then merge...
20 April 2024 1,760 0 View
I use BG 11+ Media but with an alternative trace metal mix.
17 March 2024 9,253 3 View
My protein is UFM1 tagged with Twin-Strep tag. I double-checked and my tag is not cleaved off or hidden. I made purification with IBA kit (Tactin XT 4Flow high capacity spin kit) with subsequent...
26 November 2023 4,087 3 View
Good day, I created a grid using the Autodock tools and then using those coordinates and size I created a configuration file for Vina. But in the results, the location of the ligand do not match...
12 November 2023 2,831 2 View
I am working in Paediatric Outpatient Physiotherapy department with children with Lower Limb problems. As a part of our help to children we are running 6 weeks strengthening groups. The question...
19 September 2023 2,570 0 View
I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...
12 September 2023 9,464 1 View
I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance
12 September 2023 7,596 2 View
Hi everyone. So I have performed MD simulations on desmond of my protein bound to a mutant peptide? My objective is to elaborate how the mutant peptide is inducing conformational changes in the...
21 August 2023 7,117 1 View
Hi! I ran MD Simulations of protein structure through SCHRODINGER software. The protein of interest has a lot of loops. I am getting very high RMSD of protein after simulations. How to solve this...
06 August 2023 7,344 3 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...
28 July 2024 8,169 0 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??
19 July 2024 8,571 1 View
Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...
15 July 2024 3,539 0 View
Dear all, We've been doing transesterification with our raw materials - a mixture of pairing oil (which is said to came from the skin part of a coconut) and crude coconut oil. During the addition...
14 July 2024 2,200 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View