Illumina - ECO RT-PCR. Any difficulties working with it? Is it worth the cost?

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which is better RT-PCR? How is ECO RT-PCR from Illumina? worried about its performance. Please give your valuable suggestions.. Thanking you.

Illumina - ECO RT-PCR. Any difficulties working with it? does it worth the cost?

11 December 2011 8,145 0 View

Illumina - ECO RT-PCR. Any difficulties working with it? does it worth the cost?

which is better RT-PCR both cost and performance wise? How is ECO RT-PCR from Illumina? worried about its performance. Please give your valuable suggestions.. Thanking you.

11 December 2011 4,998 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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MiRNA qpcr problem?

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Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

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Can thermal cycler machines be problematic during PCR?

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Calculating volume of DNA required from DNA concentration?

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Has anyone used the Illumina ITS1 primer sets?

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

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Valentin Penchev

Hi! I use the Eco Real Time PCR platform for several months and I really like it. The results are repeatable, the software is very user friendly and gives you a good interpretation of the results from the reaction. For now I perform only TaqMan for detecting pathogenic bacterial and viral agents, but soon I will use Eco for HRM analysis for detecting SNPs and mutations in human genes associated with infertility and I am sure that will works good. For now I have no difficulties to work with Eco and my personal opinion is it definitely worth the cost.

What type of analysis you want to perform with Eco?

Preeti Aahir

I would suggest Applied Biosystems 7500/7500 Fast Real-Time PCR as first choice and Roche light cycler 480 as second one. ABI is easy user friendly and easy to work with. Not sure about Illumina ECo though.

Satya Teja Ratnakaram

Thank u :)

Doinita Ispas

Roche has LC Nano which compares in size and cost with Eco, performances are great, tube-strip format, with pre-optimized reagents and HRM capability to state only a few things. ABI 7500 fast or not and LC 480 are bigger systems in comparison to Eco, more advanced and probably 3 times more expensive.

Eco has HRM capability too. You can perform 48 reactions (LC nano- 36) in one program. Also according to the brochures Eco is faster. May be the real advantage is a little difference in size and I am really not sure about the price, because the price of Eco is really good for such machine.

What is the price of LC nano?

Price is same as of Eco, with attractive reagent/plastic packages. "Fast" is another topic, more complex than a machine. A fast instrument with improper reagents means no benefit. Also fast cycling without uniformity is a mess. Moreover, fast reactions are not always desirable, depending on what you do. Last but not least, take into consideration the time you spend to prepare the next run - therefore you may consider pre-optimized mixes, and calculate the total time from experiment set-up to results. Nano is extremely silent and can be operated from a memory stick.