Hello! I am trying to stain CD4+ T cells for IL-17A and RORyt with no luck. I've been using the Foxp3/Transcription factor staining set from eBiosci and while the IL-17 staining looks *strictly fine* , the RORyt is always negative.
my IL-17A is APC and RORyt is BV605 if that helps.
Has anyone had any success with this staining?