Hello! I am trying to stain CD4+ T cells for IL-17A and RORyt with no luck. I've been using the Foxp3/Transcription factor staining set from eBiosci and while the IL-17 staining looks *strictly fine* , the RORyt is always negative.

my IL-17A is APC and RORyt is BV605 if that helps.

Has anyone had any success with this staining?

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