Fran Krstanović

5 Questions 3 Answers 0 Followers

Questions related from Fran Krstanović

How to efficiently isolate DNA from neurons extracted from adult mouse brains??

Hi. I’ve been unsuccessfully trying to isolate DNA from neurons extracted from adult mouse brains for further downstream analysis. First I’ve tried sorting neuronal nuclei (Approx. 105 NeuN+...

14 March 2024 8,523 2 View

Protocol for FACS analysis of Nestin+ cells from neonatal mice brains?

Hi, Does someone have experience in FACS analysis of Nestin+ cells from neonatal mice brains (PND1 to PND21)? I want to test the recombination efficiency in my NesERT2-Cre mouse line post...

02 September 2021 1,985 0 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

11 January 2021 4,164 5 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

11 December 2020 2,308 3 View

IHC marker for cytoplasmic staining of oligodendrocytes?

Hi, I'm planning on doing oligodendrocyte staining on frozen and paraffin brain sections of postnatal (up to 2 weeks old) and adult mice. I was wondering which antibody would you recommend...

19 November 2020 8,991 2 View