Usually spore formatio noccur by asymmetric division at or near the cell pole. So presumably all sporulating cells should have their endospores located at terminal or subterminal position. Then how do some cells have central endospores?
Hi I performed 24 hr biofilm formation assay (a total of nine replicates for each bacterial strain). And from the OD570nm, I classify them according to their biofilm forming capacity by using the...
07 August 2019 5,736 3 View
Hi I would like to know how to construct standard curve for azocasein assay? I know that tyrosine is usually used for the standard curve of protease assay. The tyrosine standard measured...
03 April 2019 3,292 3 View
Hi I extracted plasmid from B cereus (isolated from rice in which emetic strains r more prevalent) I am trying to detect emetic toxin (CES) gene which is located on plasmid. When I run gel to...
11 December 2018 8,146 2 View
Hello I'm trying to extract plasmid DNA from Bacillus cereus by using an extraction kit. I was told that lysozyme treatment step has to be included as well in the protocol given by the...
11 December 2018 1,300 9 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
What could be the reason for mixotrophs to be yellow too instead of green? Thank you
08 July 2024 594 2 View
I'm currently analyzing the Raman spectra of coloured plastic samples and have encountered a wavy, oscillating pattern in the spectra of some coloured samples. I was wondering what the possible...
04 July 2024 1,949 4 View