Yugenraj Navaneethan

5 Questions 6 Answers 0 Followers

Questions related from Yugenraj Navaneethan

Statistical analyses for biofilm assays?

Hi I performed 24 hr biofilm formation assay (a total of nine replicates for each bacterial strain). And from the OD570nm, I classify them according to their biofilm forming capacity by using the...

08 August 2019 5,711 3 View

What is the right azocasein assay method?

Hi I would like to know how to construct standard curve for azocasein assay? I know that tyrosine is usually used for the standard curve of protease assay. The tyrosine standard measured...

04 April 2019 3,259 3 View

What is the optimum concentration and incubation time for lysozyme treatment in plasmid extraction from Bacillus?

Hello I'm trying to extract plasmid DNA from Bacillus cereus by using an extraction kit. I was told that lysozyme treatment step has to be included as well in the protocol given by the...

12 December 2018 1,262 9 View

Plasmid DNA is not visible in gel electrophoresis?

Hi I extracted plasmid from B cereus (isolated from rice in which emetic strains r more prevalent) I am trying to detect emetic toxin (CES) gene which is located on plasmid. When I run gel to...

12 December 2018 8,105 2 View

If sporulation occur by asymmetric division, what could be the reason for formation of central endospore?

Usually spore formatio noccur by asymmetric division at or near the cell pole. So presumably all sporulating cells should have their endospores located at terminal or subterminal position. Then...

08 August 2018 7,225 0 View