If I want to perform a multiplex PCR for the target genes. Is there a software that can determine the proportions of used primers and any other factors instead of the principle of change of primers proportions that M-PCR based on it?
Dear Suzan, I don't know a software related to your problem, but my suggestion to use low concentrations for small amplicons and high concentrations for long amplicons in your multiplex PCR. The annealing temperatures should be same or close to each other. The following paper maybe helpfull.
Best regards.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, July 2002, p. 2155–2161