I want to induce oxidative stress by hydrogen peroxide in S. cerevisiae. The stress was measured by t-bar assay. But the cells are not taking stress.
Good luck
I treated the culture with H2O2 40mM for 1 hour and I could see the lipid peroxidation using T-bars. My culture were in YPGly (medium with glycerol), I don't know if the H2O2 concentration should be different in other media.
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...
01 March 2021 1,899 2 View
Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...
28 February 2021 8,189 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
24 February 2021 5,378 1 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View
In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...
21 February 2021 1,864 2 View
Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...
16 February 2021 2,575 4 View
To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...
16 February 2021 7,068 24 View
I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...
01 March 2021 10,078 1 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...
26 February 2021 3,668 1 View
25 February 2021 6,760 1 View
I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...
24 February 2021 8,105 3 View
I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...
24 February 2021 8,678 2 View
I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...
21 February 2021 8,694 4 View
Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...
11 February 2021 9,607 3 View
The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...
11 February 2021 952 6 View
Hi there, I'm having trouble with adding cyclic loading in OpenSees software. My task is to model a reinforcing bar under cyclic loading and compare the response with the experimental results....
04 February 2021 8,158 1 View