If I store tissue sample in PBS at -80 C long time, can I extract RNA from the sample?

Sarit Charuchaibovorn @Sarit-Charuchaibovorn

01 November 2013 1 6K Report

My tissue sample is a whole parasite (B.malayi) that stored in PBS at -80 C for 5 years. Can I extract RNA from the sample?

Thankyou

Ronald Blanton

If the sample was quick frozen and placed immediately in -80 at the start you should be able to. I have for schistosomes after even a longer time. Low pH is also important. As the pH approaches 7.5 or worse 8, it becomes more difficult.

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