Sarit Charuchaibovorn

14 Questions 11 Answers 0 Followers

Questions related from Sarit Charuchaibovorn

Why is L4 stage of C.elegans used in research paper for LC50 assay?

Why is the L4 stage of C.elegans used in a research paper for the LC50 assay?- The most of the paper which performed the LC50 of drug/substance to C.elegans used L4 stage, however, some...

23 April 2017 1,884 0 View

How should I store ivermectin and albendazole solution?

Hello, I have prepared the stock solution of ivermectin (sigma) and albeldazole (sigma) (in 10% DMSO) for LC50 nematode assay. I would like to know "How should I store ivermectin and albendazole...

19 December 2016 4,983 3 View

How to discriminate stage of C.elegans?

It's my first time to culture C.elegans. I saw the html link (INTRODUCTION TO C. elegans ANATOMY http://www.wormatlas.org/ver1/handbook/anatomyintro/anatomyintro.htm). They described each stage of...

22 September 2016 3,655 6 View

Is possible that the nematode gene has 5'utr up to 10kb?

Hello, I'm trying to identify the unknown gene (phosphoglycerate mutase) from a nematode by RACE technique (Clonetech, Takara)(The CDS orthrologue gene in C.elegans is 1.5 Kb ). I performed 5'...

21 September 2016 6,908 2 View

What is the difference between eosinophilic infiltration and Loeffler’s-like syndrome?

Now, I am studying about strongyloidiasis. From my literature, I found that the pulmonary symptoms of strongyloidiasis include a dry cough, pneumonitis, eosinophilic infiltration...

02 May 2016 3,143 2 View

How to calculate the synergistic index of drug combination?

Hi, Now, I plan to combine the protein toxin and commercial drugs to treat larvae. I doubt how to choose algorithm for calculation of drug ratio and synergistic index of drug combination?Thanks

27 March 2016 4,708 14 View

How to predict the domain active site of novel protein?

Hi, I will identify and characterize some genes of nematodes. I have already identified the full-length cDNA using RACE-PCR. The next step, I would like to characterize domain and motif of this...

24 February 2016 4,591 1 View

Why am I obtaining negative western blot results?

Hello, researcher I would like to ask about my western blot results Last week, I performed dot-blot analysis on nitrocellulose membrane using mouse anti his 1:2500 (1st), and goat anti mousre IgG...

20 January 2016 7,621 5 View

What is the different of dsRNA and siRNA in RNAi?

HelloI saw in many publication about the RNAi to silence target genes, However some researchers use siRNA some researchers use dsRNA. So, i wan to know What is the different of dsRNA and siRNA in...

28 April 2015 2,950 6 View

What is an appropriate technique for blood test in hookworm infections?

Hello, everyone Now, I plan to design my experiment about hookworm parasite infection. So, I want to know what i should do in blood test for hookworm infection in golden hamster. I saw in many...

21 April 2015 2,043 4 View

How can I check the large DNA sequence (in an expression vector) ?

Hi,I want to check gene sequence that is a large size (3.7kb). This gene is located in MCS of pQE9 vector (Qiagen). How many primers that i should design for check full gene ?Do i need to...

28 March 2015 7,494 5 View

What is the best RACE kit for amplify full cDNA genes ?

I want to amplify the uncharacterized gene. First step, I will aplify and sequencing full cDNA of gene.What is the best (quality and price) the RACE kit ? (First choice, invitrogen and SMARTER...

15 October 2014 1,546 4 View

Can anyone suggest a method for culturing Strongyloides Stercoralis?

I want to culture and maintain Strongyloides Stercoralis for my research project. How should I do this?

08 May 2014 9,986 4 View

If I store tissue sample in PBS at -80 C long time, can I extract RNA from the sample?

My tissue sample is a whole parasite (B.malayi) that stored in PBS at -80 C for 5 years. Can I extract RNA from the sample? Thankyou

01 November 2013 5,883 1 View