RNA was isolated from blood serum, and the obtained pellet was resuspended in Autoclaved MilliQ water, so as to make the total mixture of 20microL. I then stored it at -20oC, for half a day, before running it on AGE.

The Loading dye was Bromophenol Blue, and I loaded 10microL of sample+ 2microL of loading dye in one single well.

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