What would my sampling technique be?

I am having issues explaining my sampling technique in my methodology. I have utilised a two phase sequential explanatory design collecting and analysing the Quantitative data in the form of an...

01 August 2019 4,049 4 View

MG-63 plasmid transfection using Lipofectamine 2000?

Has anyone got any experience of transfecting plasmids into MG-63 using Lipofectamine 2000? I am currently using it to transfect three GFP-containing plasmids - one of which is 7kb and the other...

06 February 2019 3,663 2 View

What may cause the death of mice after repeated immunization with OVA + alum?

I have tried to induce an allergic response in female Balb/c mice. Mice were immunized i.p. with OVA + alum 3 times (days 0, 10, and 20), and after the 3rd immunization, mice started to die. Does...

04 October 2018 5,171 4 View

Using regepochs function to slice the eeg data collected from Emotiv Epoc+ in EEGlab?

Hi all I am completely new to Matlab and EEG, and I've encountered an issue while I was trying to slice a continuous data with no marker captured using Emotiv Epoc+. I am using Matlab R2017b...

20 March 2018 3,075 1 View

Light wavelength attracting pest natural enemies?

Now I am interesting in if any kind of light wavelength could usefully attract pests natural enemies like lady beetle, predatory bugs, hover fly, lacewing, or parasitoids. Could any one could...

10 November 2017 894 3 View

Has anyone been able to successfully extract RNA from nucleated (avian or reptile) whole blood preserved in RNAlater?

I have Avian blood samples taken on site and stored in RNAlater blood tubes. Where I run into problems in at the Prot K/Lysis step. I end up with a solid, jell-o like blob. I know it has to do...

01 November 2017 8,910 2 View

1000 fold increase compare to control in real time PCR, is that normal?

When I over expression a protein and detect the increase of mRNA of that protein using real time PCR, the result show increase 1000+ fold to mock transfection sample. Is this result common and...

26 December 2016 6,923 5 View

If one variable (moderating) is positively skewed, how do you conduct parametric testing?

* Efforts to transform the variable via log and sqrt were unsuccessful

21 May 2016 761 3 View

Should the moderating variable be correlated with the independent and dependent variable?

21 April 2016 2,994 7 View

What can be the problem for very low or non-detectable expression after lentivirus infection?

16 August 2015 5,980 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View