If I coculture PBMC and K562, E:T ratios should be calculated from the number of PBMC or NK cells in the PBMC?
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
19 June 2024 5,950 1 View
what are novel cryptographic protocols that enhance email security?
13 June 2024 7,090 2 View
Me gustaría saber qué usos tiene la base y para que ha sifdo utilizada https://www.chemnetbase.com/
04 June 2024 1,112 1 View
sex difference in patients with myocardial infarction
17 May 2024 1,321 0 View
In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...
16 May 2024 6,266 5 View
I would like to simulate the high and slow spin configuration of a metal oxide. I am using QE for it, but in my research, I found that this kind of calculus is not possible. Or if it is possible,...
15 May 2024 6,497 3 View
It is difficult, or even impossible, to find real performance metrics (transactions cost, time to transaction finality, transactions per second, etc.) on the different blockchains. In their...
13 May 2024 10,182 3 View
I am trying to establish a model of hypoxia-reoxygenation in HK-2 cells in order to determine their expression of kidney injury biomarkers (e.g. KIM-1, NGAL, etc.) via qPCR. However, I am finding...
09 May 2024 9,223 0 View
Hello all, I am currently developing a multiple-step Explicit Foot model with the final goal to create a dynamic simulation of the stance phase. To do so, based on previous literature, my idea is...
02 May 2024 9,008 0 View
Hello all, I use sensors to measure nitrogen, potassium and phosphor in agricultural soil. Normally, laboratorium tests would be done to measure this. But we use npk sensors. These NPK sensors...
03 April 2024 508 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View