If extraction reaction is unaffected by temperature then what is the reason for its proper justification?

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Can I use DEAE Sepharose in place of DEAE Cellulose?

I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...

15 January 2023 2,501 0 View

How to reduce noise in OCP and EIS study using Potentiostat?

Dear all, I have been doing trials for accessing corrosion properties of steel rebar in chloride environment using Potentiostat, wherein my working electrode is steel rebar, reference electrode is...

15 November 2022 8,797 0 View

Dear all I wanted to Ask is there are any traditional method for isolation of T Cells?

is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...

20 September 2022 1,266 1 View

Hi everyone. So I'm trying to locate nanoparticles inside plant tissues. Is there a technique I could use that can detect them without sectioning?

I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?

28 August 2022 2,370 0 View

How to use Case study method in Design Management research?

I'm conducting research to understand the design management practices in early stage start-ups. I 'm taking a action research and case study method as my approach. Would like to connect with...

14 February 2022 7,613 0 View

What type of plasticity model and its data for wood material should be input in ABAQUS software?

I am an M.Tech structural engineering student working on the project ' Numerical analysis of Kath-kuni architecture ( a common masonry typology ) in Himachal Pradesh region of India subjected to...

11 January 2022 1,154 3 View

Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,611 4 View

If we have without any reference standard how are you calculate the activity of sample in gamma ray spectrometry?

Gamma ray spectrometry

09 April 2020 5,409 0 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,205 1 View

Does anyone has any idea, how to get rid of TEA contamination in LC/MS?

We have Agilent 6300 series Ion trap in our lab. Last week I used TEA to reduce the tailing of the compound and after that a strong peak started to appear at 102 which is of TEA. Can anyone...

23 June 2019 4,072 10 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View