Upon parafilming and snap freezing a 6 well plate in LN, I noticed 2 of the wells got filled with liquid nitrogen. Are they still viable for protein extraction or should I just avoid using those 2 wells to extract protein?
Yes, you can still extract proteins from cells that have come into contact with liquid nitrogen (LN). In fact, liquid nitrogen is commonly used in laboratories to quickly freeze cells and preserve their protein content. The rapid freezing helps to prevent degradation of proteins and other cellular components. After freezing, you can proceed with protein extraction using various methods such as cell lysis followed by centrifugation to separate the cellular components, including proteins, from other cellular debris. The proteins can then be further purified and analyzed for various purposes such as biochemical assays, western blotting, or mass spectrometry.