Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How i can Improve the accuracy of an artificial intelligence model for test data in MATLAB ?

Hello everyone, I have a question about artificial intelligence in MATLAB. Can you help me, to improve the accuracy of an artificial intelligence model for test data in MATLAB software? (while...

22 July 2020 2,692 12 View

Does anyone know how can I get Mito Thermo Yellow (MTY) dye?

This is a thermosensitive dye that was recently used by chretien et al to measure changes in mitochondrial temperatures.

01 March 2020 4,863 4 View

How to continue after tissue processor failure (samples left to dry for hours)?

For some reason our tissue processor had stopped working during last night, leaving my samples (different murine tissues fixed in 4% PFA o/n) in the air before the last ABS-ethanol. I put the...

20 January 2020 523 2 View

How to use yalom existential psychotherapy or group therapy in school setting?

Psychotherapy can help most patients confront the issue of responsibility for their own life, problems, and improvement (Yalom, 1974). Group therapy can be somewhat beneficial for clients who are...

12 December 2018 6,850 3 View

What are the safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix?

Hi for All I need your recommendations from safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix. Thanks in advance

06 July 2018 326 2 View

Do you know the latest list of the journals that discontinued from Scopus?

Hi dear colleagues Do you know the latest list of the journals that discontinued from Scopus? You can see it in this file which downloaded from Scopus website (last updated 26/3/2018). Regards

14 April 2018 4,977 17 View

From the copyright viewpoint, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform?

Hi dear colleagues. From the copyright viewpoint for journals, is it legally permitted for the researcher to add the full text of the published paper on the research gate platform? This question...

05 April 2018 9,253 74 View

Is there any useful book or standard to interpret the concrete microstructure tests (for example SEM, XRD,....etc.) results?

Hi dear colleagues, Is there any useful book or standard to interpret the concrete microstructure tests (for example SEM, XRD,....etc.) results? Regards

28 March 2018 9,546 13 View

Do I need to use sequence alignment to map a variation to a genome?

A VCF file is generated through variant calling which basically uses alignment to identify the variants. If I have an unknown VCF and I know which reference genome was used to generate it, can I...

05 March 2018 4,528 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View