we are very much interested in plant genetic engineering with the view of improving salinity tolerance.
Please check the following articles it will help you
https://pdfs.semanticscholar.org/c44f/9adbb02e57d7eb773d43cde0ae29d9a1ce02.pdf
check this old protocol
http://link.springer.com/chapter/10.1007%2F978-94-009-5133-4_7
You can check also my own protocol for the Protoplast Isolation. You can try this protocol with you plant.
Hi Muayed Fadhil Abbas,
If you are doing protoplast fusion to bring 'salinity tolerance' into an elite line from a donor line, won't the resulting lines carry some undesired traits from the donor line? How will you remove the undesired traits later?
Protoplast fusion seems to have been successfully performed on cereal crops. See the 'Cereal' section of the attached paper (p.151).
Paper Tile: Plant protoplasts: status and biotechnological perspective.
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