All kits were for mammalian cells. I understand that the co-factors are the same, but yeast cells will need specific extraction step which can later interfere with kits applicability.
Suggestions for extraction/lysis are also welcome!
Hi there,
The following should be helpful:
Article Single sample extraction protocol for the quantification of ...
Hi all, Does anyone have any experience in culturing RSV and/or hMPV? What are the best cell lines for general propagation and is there are any special requirement for the lab beside general...
30 April 2020 9,627 0 View
Probability sampling I know, but it seems be different.
13 March 2014 4,385 12 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.
27 June 2024 7,473 4 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...
18 June 2024 2,106 0 View
Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...
17 June 2024 1,069 3 View
I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...
10 June 2024 5,941 3 View
Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...
05 June 2024 5,040 1 View