Please look at the image. It is showing DPPH free radical scavenging activity of something...
According to the image, what would be the IC50 value of Ascorbic acid used as a standard for that study?
Formula-
IC50 = (50-b)/a
You can use ED50 Plus v1.0 software.
This is very precise software to calculate EC50.
You can find it for free online via google search with "ED50 Plus v1.0 software".
With kind regards
Christophe
0.4
Plot a graph of the percentage inhibitions Vs concentrations used. Then extrapolate from the 50 percent down the concentration. The value you got is the IC50.
Optionally you can use SPSS software
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