Hello,
I used DPPH to estimate the antioxidant activity for a number of plant extracts. I calculated the IC50 for all of them. From literature, I found a term called antiradical power (ARP) = (1/IC50)*100. I calculated that parameter as well, then I calculated the correlation coefficient for the relationship between the total polyphenolic content (TPC, using Folin-Ciocalteu assay) and IC50 then between TPC and ARP. The correlation coefficient were totally different. For TPC and IC50, it was -0.44, while it was 0.88 for TPC and ARP. How can I explain this big difference? and which relationship is more reliable to include in my results?
Thank you
Gülüzar Özbolat, Thanks for the paper, but nothing is mentioned about which the best way to put the results. They calculated IC50 and its reciprocal in a table, that's it.
The effective concentration of sample required to scavenge DPPH radical by 50% (IC50 value) was obtained by linear regression analysis of dose-response curve plotting between %inhibition and concentrations
Gülüzar Özbolat, I calculated both IC50 and ARP, but their correlation to TPC are totally different, so which one I should use to represent my data. It will be a big question to have such a conflict in the correlations.
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