Fatma Elzahraa Mohamed Elessawy

6 Questions 17 Answers 0 Followers

Questions related from Fatma Elzahraa Mohamed Elessawy

Can ferrozine assay be modified and used for different purposes?

Hello Everyone, I am planning to use ferrozine assay to measure the chelation ability of plant extracts (containing polyphenols) by reacting FeCl2 (ferrous) with the extract of interest then add...

08 August 2018 9,301 4 View

IC50 or antiradical power?

Hello, I used DPPH to estimate the antioxidant activity for a number of plant extracts. I calculated the IC50 for all of them. From literature, I found a term called antiradical power (ARP) =...

07 July 2018 7,594 5 View

Can I use "quercetin equivalent" for a flavonoid measured by LC-MS?

I have a plant extract with lots of flavonoids there. An abundant flavonoid was identified and confirmed but its pure standard form is not commercially available. I used quercetin standard curve...

06 June 2018 4,232 8 View

Do I need to include all replicates for different concentrations in the same graph, or have a separate graph for each series of concentrations?

I am using DPPH assay to measure antioxidant capacity of my plant extracts. I diluted each sample 10 serial dilutions and I estimated the antioxidant activity in 4 replicates for each dilution. I...

04 April 2018 9,556 13 View

Is it difficult to conclude a statistical model for complex mixtures?

Hello everybody, I have some complex extracts that I am using to test antioxidant activity. I wanted to compare the antioxidant activity of an extract to a synthetic mixture with the same...

03 March 2018 6,361 10 View

Should I use the mean of triplicate or incorporate the triplicate results in the statistical test?

Hello Everybody, I measuring the concentration of certain compounds in different genotypes of a plant. I measured the concentration in triplicate using LC-MS. I am confused about using the mean of...

02 February 2018 243 3 View