Hi All,
I have conducted Ibuprofen dissolution test in phosphate buffer pH=7.2. The calibration graph was also predicted with a reasonable R^2.
However, after using UV-Vis for analysing the samples, the reading are much higher than the range of calibration graph. The samples were diluted but again after the calculations, the dissolved amount of Ibuprofen seems to be higher than what was actually added to the dissolution apparatus container.
Can you please help me understand what is the reason for this increase of the peak intensity?
Should be mentioned that this is an amorphous formulation containing excipients with Ibuprofen.
Thanks.