02 March 2022 1 1K Report

If it is possible, I would need to consider any special step to realize this kind of measurement? I will really appreciate your answers or opinions about that.

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How to convince the public to wear masks/gloves if they are resistant?

As you all know, this pandemic has taken a major toll on the entire global community. In some societies, people do abide by COVID protective measures. Yet, in other societies people are resistant...

30 September 2020 546 13 View

I am having a problem ligating my vector with PCR product by using the pcDNA3.1 V5-His-TOPO expression kit.Have anybody had such an experience?

I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert...

06 September 2020 3,483 0 View

What are these additional bands in my agarose gel results?

I have included pictures to illustrate my point. In the gel image that has only 4 lanes: Lane 1 is a 1 kb molecular weight ladder. Lane 3 is my wild type DNA (in a pGEX plasmid) and Lane 4 is the...

28 August 2020 4,146 4 View

Delta Furin SARS-COV-2 mutant Western Blot Analysis showed double band around 250 kDa. Processed and unprocessed version? Need evidence, please help?

I analysed SARS-CoV-2 Spike Protein delta Furin mutant in my bachelors thesis and need evidence (paper, research or something like that) to proof my suggestion. I think the double band at around...

26 August 2020 1,058 4 View

How to get SD from CI??

I apologize in advance for my rookie question. I have been looking in the internet how to get SD from CI values, and some sources tell me to apply the formula =SQRT(N)*(CI_upper.limit -...

19 August 2020 8,946 2 View

Who I should contact to get permission to use the BREQ-2 tool in academic research?

The BREQ-2 tool is commonly used in physical activity research to measure motivation. The tool was developed by David A. Markland. I have not been able to find how to ask consent for its use in...

15 April 2020 5,428 3 View

How to aggregate data from various surveys and reflect them onto one person?

So for example, we have the following polls: First poll: People who said they like orange juice for breakfast are as follows: Texas (50%), NY (25%), North Carolina (20%), and Florida (5%) Second...

01 March 2020 2,916 3 View

How do voter data companies collect or match info?

I have been recently studying proprietary voter files and data. While I know that voter files are for the most part public (paid or free), I am confused as to how companies match this data to...

30 January 2020 7,520 1 View

Is Bowtie2 assembly file saving problem caused by no space left on the disk?

Hello, Here is what I got with Bowtie2 while changing .sam file to .bam file: "samtools view -bS egpart1.sam > egpart1.bam [E::bgzf_flush] File write failed (wrong size) samtools view: writing to...

28 December 2019 3,244 2 View

What type of design?

I am asking for a student I'm mentoring. It's an action research project with a small sample of maybe 8-10 participants. If the student includes a likert attitude scale but is mostly focusing on...

08 June 2019 9,553 4 View

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