Need miRNA.gtf genome cordinates for counting miRNA short reads by GFOLD.
Rupesh, don't know about miRBase, but for general GTF GFF conversion, see these pages: http://blog.nextgenetics.net/?e=27
The difference is mainly in the last/attribute column. Also GTF is for genes only, while GFF3 can carry any feature defined by Sequence Ontology.
May be this is a very basic question for your all, but I was just wondering to get answer. Is it possible that one miRNA precursor with the same mature (identical) sequences has only one locus or...
05 June 2014 2,515 6 View
Need to work with Chip-seq data sets for medicinal such as Withania somnifera etc.
31 December 2013 1,489 5 View
need to check self designed pipeline for miRNA analysis .
05 June 2013 5,607 5 View
We have raw data in COLOR space format (.csfasta) and would like to profile and analyze it in order to find known and novel miRNA in sample. Further we are also looking for different expression...
03 April 2013 401 2 View
We have found a novel gene during NGS (next generation sequencing by solid run) transcriptome analysis. So let me have your suggestions regarding bioinformatics.
01 February 2013 3,859 8 View
I have build small RNA 3D strucutre by using ERNA3D and wanted to dock it with some proteins that present in PDB database.
09 October 2012 3,590 3 View
Can anyone recommend how to do this? I'm looking at protein and nucleic acid domains inside my modeled 3 D structure with already existed PDB.
03 April 2012 3,578 2 View
There are many tools but problem to build when loop is present in the microRNA and also when separate it from protein. so i want better tools for modeling the microRNA.
31 December 2011 5,625 5 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...
03 March 2021 2,870 3 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View