Need miRNA.gtf genome cordinates for counting miRNA short reads by GFOLD.
Rupesh, don't know about miRBase, but for general GTF GFF conversion, see these pages: http://blog.nextgenetics.net/?e=27
The difference is mainly in the last/attribute column. Also GTF is for genes only, while GFF3 can carry any feature defined by Sequence Ontology.
May be this is a very basic question for your all, but I was just wondering to get answer. Is it possible that one miRNA precursor with the same mature (identical) sequences has only one locus or...
05 June 2014 2,592 6 View
Need to work with Chip-seq data sets for medicinal such as Withania somnifera etc.
31 December 2013 1,584 5 View
need to check self designed pipeline for miRNA analysis .
05 June 2013 5,692 5 View
We have raw data in COLOR space format (.csfasta) and would like to profile and analyze it in order to find known and novel miRNA in sample. Further we are also looking for different expression...
03 April 2013 476 2 View
We have found a novel gene during NGS (next generation sequencing by solid run) transcriptome analysis. So let me have your suggestions regarding bioinformatics.
01 February 2013 3,968 8 View
I have build small RNA 3D strucutre by using ERNA3D and wanted to dock it with some proteins that present in PDB database.
09 October 2012 3,668 3 View
Can anyone recommend how to do this? I'm looking at protein and nucleic acid domains inside my modeled 3 D structure with already existed PDB.
03 April 2012 3,664 2 View
There are many tools but problem to build when loop is present in the microRNA and also when separate it from protein. so i want better tools for modeling the microRNA.
31 December 2011 5,719 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View