Does anyone know why I have got multiple unclear bands image of a western blot?

I'm doing western blot to detect LRRC8A protein which is extracted from colon cancer cell HCT116, unfortunately, I could not find a good result. Does anyone have an idea or advice?

21 November 2019 2,106 3 View

Can an ELISA assay for glucocorticoids (GCs) measurements be used in assaying GCs in tissue lysates?

I would like to measure GCs in tissue samples and I have tissue lysates. Is it feasible to use an ELISA-based assay or is there a better alternative? Thanks in advance!

04 April 2018 5,578 1 View

Is SILAR technique can be useful for preparation of 2D monolayers? e.g. boron nitride?

Is SILAR technique can be useful for preparation of 2D monolayers e.g. boron nitride?

14 March 2017 5,421 1 View

Is there an exact method to sub-fraction plants extract ? Does it differ from one plant to another?

The plants are from lamiaeae family

15 May 2015 9,351 5 View

Is there a protocol to sub-fraction the extracted materials from a plant?

does this protocol differ from plant to another

14 May 2015 8,454 7 View

What is the main risk of password authentication in cloud computing?

This field considers the heart of security world.

07 May 2014 8,414 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View