I am staining my cells with DAPI and Hif 1 alpha and I want to measure the nuclear translocation of Hif 1 alpha under hypoxic conditions by image J in confocal Images. how can i do that.
I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...
26 June 2024 5,049 5 View
Salam, Hi Respected Researchers and Fellows, I'm currently perusing my Masters Degree in National College of Arts in Lahore, Pakistan. I’m working on my Thesis, My research is to Bridge the Gap...
30 May 2024 6,903 0 View
Types of Agricultural Waste: Crop Residues: Stalks, straw, leaves, husks, etc. Animal Waste: Manure, bedding, slaughterhouse waste. Processing Waste: By-products from food processing like peels,...
27 May 2024 3,851 2 View
when data is not normally distributed which nonparametric test to be used if you have 2 groups experimental and control group with 3 levels pre post and follow-up also, can we use parametric test...
20 April 2024 4,707 1 View
I am studying leadership style's impact on job satisfaction. in the data collection instrument, there are 13 questions on leadership style divided into a couple of leadership styles. on the other...
16 April 2024 4,784 3 View
why multivariate test table shows non significant value whereas univariate shows significant value
27 March 2024 9,269 4 View
my research was intervention with 2 groups experimental and control group with dv 1 with five subscales one's value should decrease others value should increase has 3 levels pre post follow up, i...
20 March 2024 6,115 5 View
What is the real homepage URL of migration letters journal ? its Scopus and impact factor and publisher details
19 March 2024 7,172 4 View
I have done optimization of different ionic liquids ,and polymer and ionogel by DFT . I have found the different thermoelectric paramters of these . I would like to know how to select the best...
02 March 2024 9,166 0 View
Why does the inner stream in the vortex tube lose heat and the outer stream gain heat in R-H vortex tube. I am looking for an explanation that describes the physics of the process.
15 February 2024 2,225 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View