i want to know about the dose of the scopolamine to induce memory loss in mice? can anyone share good papers about the scopolamine induce memory loss.
how can i draw Stacked view of HPLC chromatograms? please suggest good software for it.
10 November 2018 1,994 5 View
i want to make a chromatogram like attached file. please suggest me how can i draw it offline?
10 November 2018 3,538 0 View
i want to induce iron overload or deficiency in mice without iron diet. in short period of time like in a week.
06 July 2018 8,314 0 View
i have extracted the DNA from mice ear by NaOH method. some times it works but sometimes doesnt wokr. i used 50um NaOH 100ul . heated at 98 temp for 30 mins. then added 10ul Tris Hcl pH 8. then...
06 July 2018 9,879 6 View
i want to culture Brain microvascular endothelial cells for my project. but i want to culture by easy method . or can i use collagen type 4 instead of 1 ?
05 June 2018 6,525 4 View
after culturing for many days brain microvessels endothelial cells can survive ?
05 June 2018 4,983 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View