Dear Ajeet Singh

You can do the analysis using GC-MS if it is non-polar fraction and you are comparing with some know compounds in literature.

Sine it is methanol fraction (polar fraction); one need to do LC-MS and compare with known compounds database.

If you are expecting a new compound then the other method is activity guided fractionation of crude extract followed by identification of purified compound using IR, NMR, MASS, CHN etc.

Dear Ajeet Sing,,

Identification of unknown compounds from the crude methanolic plant extract may be done using GC-MS analysis (qualitative) by comparing data with the NIST & Wiley library..

Thanks, Sir. 

Dear Ajeet Singh

You can do the analysis using GC-MS if it is non-polar fraction and you are comparing with some know compounds in literature.

Sine it is methanol fraction (polar fraction); one need to do LC-MS and compare with known compounds database.

If you are expecting a new compound then the other method is activity guided fractionation of crude extract followed by identification of purified compound using IR, NMR, MASS, CHN etc.

Dear Madam,

How I identify/confirm unknown compounds from extract fraction using LC-MSMS as there NIST & Wiley library is absent ?

Thank you, Madam, Madhu Chopra for valuable information.  

I think this was asked in two places? Please see my answer in the other instance of this question...literature search on you plant.

I recommend you LC-MS, I hope you have a data base to compare.

By other side I suppouse you did a bibliography Research to know some about your plant at least about the genus which your plant correspond. Good luck

Please  read  the attached file .

Thanks, Harish Andola sir for your valuable information. 

• if you any help my side  please Email me: [email protected]

Okay Sir, Thank you.  

It depends on the the fraction, nonpolar, or polar, volatile or nonvolatile, you can use the GC-MS with the NIST  or LCMS with the library to compare,

thanks, Hanna M. Madanat sir 

It depends on your plant extract because the every test materials by LC-MS or HPLC, GC-MS give different, molecular weight and structure of the components .

If you want to identify unknown compounds, which is absent in library, the best will be LC/MS/MS with TOF or Orbitrap. They have special software for identity new compound.

If you have idea what group of substances you have, you should check library (NIST or similar) and try to use coumpound standard.