I want to do a GC- MS analysis for Prodigiosin pigment extracted from Serratia. It's boiling point is 554.3 Celcius. Should I use headspace method?

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What is ideal volume for head-space in the semi-continuous anaerobic digestion? Is 30% of Total Volume of the reactor recommended?

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George Danila

For this compound with such a high boiling point, its more suitable to use LC/HPLC coupled with MS. Or if you can find a derivatization agent to lower the boiling point maybe you can see it on your GC-MS. 554C is not a achievable temperature for GC oven or Headspace unit.

Kavindi E. Weerasinghe

George Danila Thank you sir!