My dear the following link will help you:

https://www.scientistsolutions.com/forum/biochemistry-general-biochemistry-discussion/difference-between-tris-cl-tris-hcl

Hi,

higher difference between this buffers . Normally the Tris HCL buffer is alkanic while HCl buffer is acidic

Hello Reena

The goal of a buffer is to keep the pH of a solution within a narrow range. While the ratio of [A-]/[HA] influences the pH of a solution, the actual concentrations of A- and HA influence the effectiveness of a buffer.

Hydrochloric acid on it’s own cannot act as a buffer because it is a strong acid and dissociates completely in aqueous solution. However, hydrochloric acid can produce a buffer solution when mixed in the right proportions with the salt of a weak acid. Tris (as a pure compound usually supplied) is a base and its aqueous solutions are alkaline. It is not a buffer. To acquire a buffering capacity it sould be in equilibrium with the corresponding acid which is the protonated form of Tris. Traditionally, protonation of Tris is accomplished by addition of HCl. pH of the solution decreases to the desired value and the resulting Tris-HCl solution is what we commonly call Tris buffer.

Hope this helps, good luck

Thank you Himani mam for sending of description related to HCL and Tris-HCL buffer.

I am sending u one reference which i want to follow for my research work, that mentioned HCL buffer.

Korakli et al.2002

Dear Reena, could you please send full reference so that I can find it easily.