Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

How do I organize items to repeated surveys for quantitative and qualitative analysis?

Hello fellow researchers! Newbie here :) I created a survey with open-ended and Likert-type questions. The survey was administered nine times to the same people using google forms. I plan to...

01 January 2021 3,921 3 View

Is there a better library or function for plotting CFA with many observed variables in R?

Hello! I'm kind of new in using R for doing EFA, CFA, and SEM. I just followed a guide to perform CFA on my data to see how my context fits the ITC-SOPI model in a VR photo-based tour. I used...

23 December 2020 1,679 2 View

Best way to validate CRISPR-Cas9 editing efficiency?

I'm trying to obtain genetic KO in my cell line of interest using Lentiviral-mediated CRISPR-Cas9 delivery system. I would like to test more than one unpubblished sgRNA targetting a couple of...

10 August 2020 1,324 4 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

Fluorescence labeled antibody?

Hi all, I have a small question for you. Is there any fluorescent labeled antibody available which emits the fluorescence only if bound to substrate? So the unlabeled version does not emit any...

19 March 2020 5,715 2 View

How to evaporate Methyl Acetate, and purge added Ethanol?

To use an inhibitor, prepared and sold in a methyl acetate solution, I need to evaporate the aforementioned solvent and exchanging to ethanol or DMSO. I will do that with a gentle nitrogen stream....

01 March 2020 6,947 3 View

SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

08 January 2020 3,526 3 View

How to solve multicollinearity problem in mixture design?

Due to its own nature mixture design models (mainly with interactions) presents multicollinearity problems, some authors declare that a combination of forward stepwise and step backward stepwise...

05 January 2020 1,136 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Is this moth a Diatraea saccharalis?

The caterpillars of this moth were found in stems of Echinochloa polystachia grass as stem borers. The length of the caterpillars are something about 1.7 cm. I have compared images from the...

24 February 2021 309 3 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View