I treated cells with a drug now i want to separate drug from cell components. i want to pellet down total RNA,DNA and protein how i can ?

More Firoz Bhati's questions See All

How many ways we can calculate the Theoretical diametral Stiffness of a pipe?

What are the different methods of calculating the theoretical diametral stiffness of a pipe? If possible, please share the citations. Thank you so much.

28 October 2023 3,093 1 View

How to draw a significant lines in volcano plot in excel?

Particularly, I want vertical significant lines which show upregulation and down regulation of expressed proteins.

19 August 2023 9,826 0 View

How to calculate the Radial Distribution Function in TRAVIS software by using XDATCAR File?

I would like to calculate the radial distribution function by using XDATCAR file which is generated by Ab initio molecular dynamics using VASP. I am trying with TRAVIS software but I am not able...

25 June 2023 3,985 0 View

How to narrow down a topic for phd research in the area of marketing?

I am a research scholar. The area i choose for phd is Management (Marketing). My supervisor suggested me some key words to find literature, which are (Big Data Analytics and Sustainability)....

30 May 2023 1,143 4 View

Why I-V curves obtained from SCAPS 1D simulation are sometimes bending outwards at open circuit condition?

I have used simulation parameters from the published papers for cell structure Au/Spiro-MeOTAD/CsFAPbI3-xBrx/SnO2/FTO but the curves are bending for various thicknesses of HTL. Also, in some...

16 October 2022 1,034 0 View

Why conversion error in SCAPS 1D simulation?

When I try to simulate perovskite solar cells using SCAPS 1D then found a conversion error for the NiOx HTL layer. How to solve this issue?

16 October 2022 3,004 7 View

How can we increase humidity in a mushroom shed?

Oyster mushroom

21 April 2022 9,576 7 View

Where do we get the -ve strand (with reverse direction) DNA sequence of Human genome ?

I need to find out the occurrence of specific sequences across the genome in both strands. I'm able to retrieve the FASTA sequence of the +ve strand from the NCBI reference genome. How to get...

14 March 2022 8,342 4 View

Is there any convenient method to dissolve super absorbent polymer (sodium polyacrylate) ?

I want to dissolve the superabsorbent polymer. I have tried dissolving it in distilled water and methanol but it was insoluble (gel form).

05 October 2021 1,586 3 View

Which software are suitable to extract the current contribution of a solar cell using upconverters or downconverters?

I want to simulate the current contribution in the perovskite solar cell using photon upconverters and downconverters (as attached file). Which software is suitable for this type of simulation? Is...

08 May 2021 8,713 3 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View

MTT assay - 6 well plate?

Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...

13 July 2024 9,783 1 View

How to convince decision makers to adjust the legislation to allow opening of drug consumption rooms?

In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...

08 July 2024 8,903 1 View

Is the use of microcrystalline cellulose MCC 102 in direct compression technology justified, given the low flowability of this excipient?

Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...

08 July 2024 2,807 0 View

What are the trending topic to writ e a review article on it?

The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field

02 July 2024 4,031 3 View