Indomethacin is a non-steroidal anti-inflammatory drug (NSAID) that is commonly used to treat pain and inflammation. However, at high doses, it can have significant side effects, including death.

The reason for the deaths of the rats in your experiment could be due to toxicity from the high doses of indomethacin. NSAIDs can cause toxicity by damaging the gastric mucosa, leading to ulcers and bleeding, and by affecting the renal function, leading to kidney failure. High doses of indomethacin can also lead to central nervous system (CNS) toxicity, which can cause seizures, coma and death.

Additionally, there could be a number of other factors that could have contributed to the deaths of the rats in your experiment, such as:

• Variability in the rats' susceptibility to the drug: Some rats may be more sensitive to the effects of indomethacin than others, which could explain why some rats died while others did not.
• Variability in the administration of the drug: The method of administration, such as oral, intraperitoneal or intravenous, can affect the bioavailability and toxicity of the drug.
• Other health issues: The rats may have had pre-existing health issues that made them more susceptible to the toxic effects of the drug.

It's important to conduct control experiments and to use appropriate dosing and administration techniques in order to minimize the risk of toxicity and to ensure that your results are reliable. Also, you may consider using lower doses of indomethacin and/or using different animal models to study the effects of the drug.

salam

What solvent do you use for indomethacin?

in first expirement i use normal sline and in second and third group i used phosphate puffer sline

hello

The solvent of indomethacin is DMSO and it is lethal to living organisms

Please make sure of this