Does anyone have experienced hanging drop method?

I've done hanging drop with A549 cells. (10 ul/20.000 cell/drop) At second day I saw a few cells have attacht on lid, behind the sphere colony. At third day they become much more cells that...

15 January 2018 4,930 11 View

How can I change number of my kernel density estimation values without changing 'std'?

Hello eveyone, I have mean value is 60, and standard deviation is 10. I'm generating 50 and 800 samples to see kernel. From kernel density estimation in Matlab, I'm getting high 'std' like 20ish...

25 June 2017 8,038 1 View

What are the best conditions for sonication for ChIP assay?

I'm going to use abcam's ChIP assay protocol. At the sonication step it just talk about times. (5-10-15 min. etc.) But there's nothing about power or cycle. What do you prefer for 500-600 bp dna...

30 August 2015 2,944 3 View

Out-of-Sample Period: Testing vs. Validation sample?

In forecasting applications, there are different strategies for sampling based on the method used. In computer intelligence, scholars need to define a longer out-of-sample period and have both...

17 November 2014 2,925 1 View

What are the storage conditions for PBS-Azide-TAME buffer?

16 June 2013 2,717 1 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Is there a way to use waveguide ports for circular antenna design in CST?

Hi, whenever i use circular array i get this error "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solver" Does anyone use circular array with waveguide ports?...

09 February 2021 3,609 8 View