I seek for control positive DNA for any of (Giardia spp., Cryptosporidium spp., Entamoeba spp., Blastocystis spp. or Toxoplasma gondii) in Egypt ?
short answer
13 June 2024 7,368 1 View
Short answer
22 April 2024 665 0 View
I will be very grateful for this kind act. My email: [email protected]
20 April 2024 957 4 View
mutations play a critical role in driving the adaptation of microbial populations to diverse environments, allowing them to thrive in a wide range of ecological niches.
04 April 2024 8,855 3 View
29 March 2024 9,596 0 View
the model i use contains independent variable (higher order construct) has three dimension (lower order construct), mediator and dependent variable. Applying reflective reflective higher order...
27 March 2024 3,984 2 View
short answer.
29 February 2024 390 3 View
As i received a comment from a journal saying: Could you please add justification on why a cross-sectional approach is appropriate here?
27 February 2024 9,459 5 View
Short answer.
12 February 2024 344 1 View
12 February 2024 7,047 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View