03 January 2025 1 7K Report

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More Kim Bedes's questions See All
Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

Is it appropriate to apply the COSMIN guidelines for evaluating the quality of tools used to assess acute postoperative pain?

Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...

10 July 2024 3,048 1 View

Similar questions and discussions
Do you know any references for analyzing stochastic fiber orientaion composites ?

Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...

04 August 2024 3,461 3 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

"Idrisi Selva" tutorial Books for pridiction with driving factors in modeling?

Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...

28 July 2024 4,168 0 View

Critique Calculaltion in Topological Indices and QSPR Modeling?

The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...

24 July 2024 9,644 1 View

How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View

Dear researchers, how can we download this book? Please help?

Several Complex Variables and Integral Formulas Dear researchers, how can we download this book? Please help?

22 July 2024 4,120 4 View

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