Did anybody use VISIONlite ColorCalc Software for liquid samples color analysis?

I would like to read about your experiences in the measurement of the color of liquid samples (wine, oil, or whatever) using a glass cuvette to calculate L* a* and b* parameters with the...

08 February 2021 6,619 4 View

My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

What is the best washing procedure for Gelatin - arabic gum capsules?

I am preparing microcapsules from complex coacervation procedure to encapsulate an oil phase. However, filtration to remove the aqueous phase results in a paste of aggregated particles. So I am...

25 October 2020 3,152 3 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

No title

26 July 2020 2,893 4 View

Based on your experience, what is the best 3D printing material for teaching human anatomy?

Dear colleagues, We all know that, ideally, human anatomy is better assessed in cadaver model. Artificial 3D-printed moldes, however, emerged to contribute to science. In order to develop...

02 June 2020 7,313 1 View

Synchronous or asynchronous classes during COVID-19 pandemic?

Dear colleagues, what is your approach for remote education during COVID-19 pandemic in terms of timing? What do you prefer? (1) Teaching live with classes pre-established at certain date (e.g....

21 May 2020 6,981 18 View

WRF-CHEM configure start and end of eruption and fall-out?

Hello, I'm using WRF-CHEM to simulate the Etna plume and fall-out. I'm using prep_chem_search.inp to input starting data, using Values.txt tu configure the height, duration, ash mass, and SO2 mass...

19 April 2020 5,503 1 View

Murine alpha-synuclein overexpression in PD mouse model?

I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...

26 March 2020 4,022 3 View

Improve GPU usage in namd?

I just installed a new RTX 2080 on an old pc (I7 980x 12 CPU cores), downloaded and installed the latest nvidia drivers and CUDA toolkit for ubuntu, also I downloaded the namd build with CUDA...

30 July 2019 375 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

ITS primers for fungal characterization?

For example, I need to characterize Rhizoctonia and Trichoderma simultaneously. In literature they are giving ITS1 &ITS3 for Trichoderma and ITS2 &ITS4 for Rhizoctonia. In our lab there is...

19 January 2021 4,157 3 View

Culturing Peziza sp?

Hi everyone, Any successful trials for getting some cultures from Peziza Fruitbodies? We have done several trials using usual medium of PDA/ MA, but all failed. Thanks in advance

29 December 2020 6,966 6 View

Is there an alternative for the enzyme mixture Driselase (from Basidomycetes) for Fusarium protoplast generation?

Dear all, is somebody familiar with an alternative to the enzyme mixture Driselase (from Basidomycetes) for Fusarium fujikuroi and/ or Fusarium graminearum protoplast generation? Were are using...

16 June 2020 5,865 2 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

Is there a catalogue of Trichoderma isolates? How are these isolates given names?

Hello everyone, I've been trying to find out how certain isolates are given a name and where to find a list of established names. e.g. there's T. asperellum T54, T. spirale T4, T. hazarnium T40...

10 April 2020 3,862 3 View

Waters ACQUITY UPLC - Issues with PDA?

I'm attempting to use a Waters Acquity UPLC prior to mass spec and I'm seeing really weird chromatograms. When I inject water, the PDA detector shows a steadily increasing signal (looks like a...

01 April 2020 4,117 2 View

How can I determine a bacterial colony forming unit?

I got samples from chicken gizzards fed with different oyster mushroom, and streak inoculated them on PDA plate after 10-6 serial dilution, now how can I determine the bacterial colony forming unit?

02 March 2020 4,823 3 View

White fluffy growth at the base of Soyabean stem?

Good day, May you please assist me with the following: What is the white fluffy growth on the base of the soybeans stem(right at the bottom on the ground). It is very common, and plants are...

01 February 2020 6,339 5 View

Is there any specific method for fungal colony growing for DNA extraction ?

I want to extract the fungus isolate DNA for Sequencing and Identification as i have different isolates from fruit peel, so i want to know that is there some requirements for colony grow...

12 January 2020 8,128 3 View