I observe yeast sediment/precipitate/aggregate very fast in the media, what could be the reason and solution?

08 December 2024 1 5K Report

I am working on yeast surface display library. After I work with yeasts for a while, I obviously see them at very bad conditions.

If I let the media stand for 1 min, I see cells aggregate very fast, form weird turbid media, and precipitate to the bottom super fast.

I assume it might be because the freezing with DMSO was harmful? But I honestly followed every step from a published protocol, so what could be the reason and what should I do?

Jorgaq Pata

The DMSO should be fine, unless you are putting tons of it, but still it shouldn't cause aggregation. What do you have in your media? There are some chemicals known to trigger yeast flocculation, or maybe the genetic background of your strain or your expressed protein does it.

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