validation
IHF methodology
Siendo trabajadora en una asociación de personas con discapacidad, en la actualidad estoy inversa en una investigación sobre "La mujer con discapacidad intelectual en el ámbito laboral" partiendo...
10 March 2024 9,266 2 View
Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...
22 February 2024 8,052 2 View
I need references on how to comment a quotation in academic paper and kinds of comments.
26 October 2023 5,364 1 View
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31 August 2023 2,566 2 View
How do I delete a publication that has already been placed on the platform?
09 April 2023 2,170 2 View
More and more adults are sharing content and photos of their children on social networks, but will this have negative consequences? It's a fashion? 📷
02 March 2023 2,473 2 View
Let's suppose there's an ecology investigation going on. The most relevant species of insects in a mountain range should be sequenced because they want to make a phylogenetic tree using...
16 October 2022 4,954 3 View
We want to test a compound against H. pylori, but it has been challenging to find the mouse-adapted strains.
01 June 2022 7,208 2 View
When calculating PLS consistent algorithm with SmartPLS, some path coefficients could be higher than 1. Why is this? Is there any problem? Does it have a solution?
22 May 2022 1,235 2 View
I have generated an Illumina Sequencing Library by nested PCR and size-selected from agarose gel. When measured with Nanodrop, the DNA is clean and of perfect quality. When sequenced with Sanger,...
05 May 2022 4,764 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View