I Need to extract plasmid DNA using non commercial methods for Antibiotic resistance from MRSA without use of lysostaphin. Anyone has experience?

How to confirm the site-directed mutagenesis result without performing NGS?

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Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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How to isolate lymphocytes from mouse spleen?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

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Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

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Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

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Alireza Mordadi

Use liquid Nitrogen for mechanical breaking cell walls.

Good luck.

Muneeba Wali

Alireza Mordadi can you please tell how would use liquid nitrogen for mechanical breakdown

Put your cryo pellets in Liquid Nitrogen and waite till your cryo completely freezes then put it in boiling water.

Repeat this.