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Questions related from Margarita Bernal Cabas
Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...
18 January 2021 9,165 3 View
Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...
27 November 2020 2,484 2 View
Hello!, I want to assess differences in the levels of secretion levels of soluble proteins in Bacillus. I am trying TCA precipitation but the concentration is quite low. I was wondering which...
26 May 2017 4,878 7 View
Hello everyone! I am a Gibson Assembly beginner. I just got the sequencing results back from my cloning and they are perfect except for one thing- I have an extra histidine (7x). I made the...
06 May 2017 5,395 3 View
Hello! I am a newbie when it comes to protein purification. I inserted a x6 his tag but when I got my sequencing somehow I ended up getting a x7 his tag. Is it a huge deal that I have an extra...
06 May 2017 6,868 3 View
Hello! I have been trying to dissolve a 5M NaCl solution but I am having issues after 4M. I have been mixing it slowly for hours now. Any suggestions on how to prepare this chemical? Cheers
07 July 2016 9,333 22 View
Hello all! I am currently trying to clone a 120bp fragment. However, I am getting a low yield. I am using 2ul of 50ng DNA for the PCR reaction. I have also tried a gradient and I am using the best...
14 March 2016 6,520 9 View
