I have H&E stained hippocampus sections and would like to look at thickness and number of degenerated cells of hippocampus at different areas in different studied groups to confirm neuronal loss after injury. Thank you
Hi Dear Somaia Fahd,
Many thanks for your question.
Please before making an original experience, study the state of the art in this field. In the next, you will found some articles, that may be useful.
Hello Dear ..,
Thank you very much for your question.
Please, before doing an original experiment, study the state of the art in this area. In the next you will find some articles, which may be useful.
I hope I answered part of your question !
Good luck !
Cordially !
Hi,
Please go through the following papers:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410525/
https://www.sciencedirect.com/science/article/pii/S0920121118302201
After the H&E staining, you can import the images in ImageJ software and use the cell counter plugin to do the counting.
I hope this helps
All the best,
Alpana
Try to stain your neurons with an anti-MAP2 antibody.
I have performed a hypothesis test (Kruskal-Wallis) and estimated the p-value using Monte Carlo. My sample size was 500. Now, I am trying to keep simulations fixed and only changing sample size to...
10 April 2020 3,950 1 View
In the attached file i want compare K2 in production sheet with A4 TO A31 in data entry sheet, and return the vaue which is in next row in matched colunm. for example in attached example....
22 January 2018 1,208 3 View
Hi! Im performing a westren blot, but my immunoblotting isnt going well. The proteins transfer perfectly on the PVDF membrane, but when I incubate the membrane with my primary abs, the signal...
18 July 2014 854 12 View
Arthritic induction is required by the protein rich in joints.
08 June 2014 5,939 4 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...
28 February 2021 7,800 6 View