I have H&E stained hippocampus sections and would like to look at thickness and number of degenerated cells of hippocampus at different areas in different studied groups to confirm neuronal loss after injury. Thank you
Hi Dear Somaia Fahd,
Many thanks for your question.
Please before making an original experience, study the state of the art in this field. In the next, you will found some articles, that may be useful.
Hello Dear ..,
Thank you very much for your question.
Please, before doing an original experiment, study the state of the art in this area. In the next you will find some articles, which may be useful.
I hope I answered part of your question !
Good luck !
Cordially !
Hi,
Please go through the following papers:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410525/
https://www.sciencedirect.com/science/article/pii/S0920121118302201
After the H&E staining, you can import the images in ImageJ software and use the cell counter plugin to do the counting.
I hope this helps
All the best,
Alpana
Try to stain your neurons with an anti-MAP2 antibody.
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