I need Rickettsia strain genomic DNA to use it as positive control for PCR assays. Can anyone tell me where I could get it?

More Sathish Sankar's questions See All

What are the procedures for environment friendly and effective methods to prepare silicon QDs, Si nanotubes and silicene (2D materials)?

There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...

10 January 2021 3,418 1 View

Understanding the true stress-strain curve of polymers as an Abaqus input?

The attached image is the measured tensile strength data (ISO standard) for commercially-available laminating epoxy (cured with hardener). Measured using a universal tensile strength machine in a...

14 December 2020 7,370 4 View

How applicable can a fertility model based on EC, pH and P be used in digital farming?

Role and function of using a developed model using three parameters - EC, pH, and P and showing its applicability.

30 August 2020 3,835 2 View

Kindly help me anyone. How photonic crystal influence the antenna characteristics like gain, directivity?

Need a detailed theory regarding, effect of PBG to increase the gain, with less directivity in microstrip patch antenna ...

03 July 2020 4,384 1 View

How to increase the gain and directivity in the microstrip patch antenna?

In CST, while designing the antenna, the antenna parameters return loss and VSWR is good. But the gain and directivity is not attaining as expected level. How to improve this?? Kindly suggest me...

23 June 2020 5,379 11 View

Any Suggestion For Custom Antibody Development?

My lab is currently looking at creating a custom antibody. There aren't a lot of customer reviews on the websites for these things, so what are the companies that you all prefer to synthesize your...

24 April 2020 1,365 4 View

Can anyone help me in this error in Unisim/Hysis? Why is the extraction column not converging?

I have used appropriate references for all the values and inputs that I have used for simulation. Please kindly help me out. I have been working through this for quite sometime. I used unisim for...

01 April 2020 773 2 View

MIKE 21 Illegal Storage Access?

Has anyone ever received the error 'Illegal storage access Abnormal completion' from MIKE? I am using the Data Extractor FM and at certain times this error comes up. Once I receive this error I...

24 March 2020 2,518 3 View

Design of equipment used for membrane distillation?

Has anyone done any work about membrane distillation? If yes please tell me about the membrane that you used and the design of the equipment and it's measurements. Please tell us the factors that...

09 February 2020 2,971 3 View

How is hardness of the ceramics measured?

As per the definition, Hardness is the resistance to the deformation. But, deformation can be seen in plastic materials only. ceramics are brittle in nature. So, the material will break when it...

24 January 2020 4,588 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View