I need protocol of Nitric oxide synthase (NOS) activity by spectrophotometer?

More Sunita Kataria's questions See All

How do I get good bands after PCR of gDNA from bacterial cells?

I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...

21 May 2024 8,614 4 View

We synthesized a catalyst by mixing L-proline and taurine. we're puzzled about the catalyst's structure. Please help to find structure of catalyst?

"As researchers, we synthesized a catalyst by mixing proline and taurine, which exhibited significant reactivity in heterocyclic compound synthesis. Characterization via proton and carbon NMR, as...

17 April 2024 8,005 4 View

Please suggest few trending & futuristic PhD Research Topics/ Gaps in Management (preferably Service Quality Management) in Higher Education in India?

Please suggest a few trending and futuristic PhD Research Topics/ Gaps in Management (preferably in Service Quality / Quality Management) in Education (preferably in Higher Education) in India....

08 April 2024 3,664 3 View

Does Nanodrop Concentration depend on the volume of the sample taken?

I checked the nanodrop conc of the plasmid sample and it was 1000 something. I cleaned the platform and the upper arm got mistakenly lowered and it still measured the conc around 41.Could be due...

14 January 2024 8,323 6 View

When the data are provided in the median and IQR, How do we calculate proportion to perform pooled analysis?

The datasets are provided as medians and interquartile ranges. Can we perform a pooled analysis? How do we convert variables to get the events as (N)?

09 January 2024 5,348 2 View

How can we introduce restriction sites in the plasmid using PCR?

If my plasmid doesn't have Ecor1 and sal1 but i need my plasmid to have those, I am aware that i can make primers with Ecor 1 and sal1 overhangs but how is that done from the beginning?

11 September 2023 2,510 3 View

Why is ampicillin and kenamycin antibiotic resistance incubation time are different? Why are sattelite colonies seen around the main colony ?

Does it have something to do with the fact that ampicillin acts only after the cells divide and kanamycin resistance has something to do with the ribosomes and then it inhibits the translation? I...

29 August 2023 4,997 1 View

Would it be wise to use the same plasmid for transfection after maxiprep if it got some water spilled in it?

Sgv was constructed and maxiprepped. The conc was 1965 ng/ul and accidently a few drops of nuclease free water got spilled in it. Can I still use the same SGV plasmid for transfection or should I...

29 August 2023 9,351 5 View

What went wrong?

I followed maxiprep protocol and the yield was as low as 5.9ng/ul. All the steps were followed and everything was taken care of. Is it because the cultures were a month old?

21 August 2023 2,773 5 View

How to convert Lithium atom to Lithium ion in Atomistic Tool Kit?

when we do DFT calculations for Li ion battery in ATK, then li ions or li atoms are intercalated???

18 August 2023 5,023 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What is the best conditions for formation Magnetite ore deposits?

I want to know more about Magnetite deposits.

06 August 2024 1,066 0 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

Does soybean seed coat or cotyledon contain chlorophyl or flavonoids? What types? determined by paper chromatography? other methods (high school lab)?

Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.

25 July 2024 7,498 2 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

Is defatted soybean considered a pulse?

According to various research, soybean is not considered a pulse because of its high fat content.

30 June 2024 6,311 0 View

What is the most accurate way to determine the energy gap of semiconductor materials? Is it better to use UV-Vis or PL measurements?

When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)

26 June 2024 7,505 6 View

What type of paraffin is recommended for preparing samples for cutting with a microtome?

Sample type: Corn leaves and stem Bean leaves and stem

22 June 2024 2,082 3 View