Has anyone compared supervised classification methods using google earth engine?

Good Morning! Has anyone compared supervised classification methods using Google Earth Engine? Could you comment on what are the criteria you used to decide on any method?

29 November 2020 544 4 View

Conv2D based CNN architecture throwing errors when importing LFW dataset in grayscale?

Hi, I am doing a research project related to Face Recognition and using LFW dataset. If I import LFW as colour = True using some additional parameters (see attached word document), the CNN...

13 June 2020 7,858 3 View

What is the best algorithm for real time pattern matching for motion identification?

Real time motion identification poses real challenge, the need for fast and light algorithm to be able to almost instantaneously identify motion being performed based on motion detected by Kinect2...

02 September 2018 5,294 3 View

Could anyone provide me examples of research opportunities in Cloud Computing and Software Defined Networking (SDN) together?

Could anyone provide me examples of research opportunities in Cloud Computing and Software Defined Networking (SDN) together? How could I work or conduct experiments using both? Articles are also...

19 January 2018 4,213 3 View

Multitemporal landsat image?

29 October 2017 2,469 7 View

Does anyone have a good publication that summarizes the crucial steps for concentration of proteins using a vacuum concentrator? Thanks!

The publication should not be a manual of a distributor.

09 November 2015 7,761 1 View

How can I access fluorescence chromatograms in Jasco's Borwin Chromatography software?

During the performance of an HPLC run, I can follow the on-line DAD chromatogram in Jasco's Borwin PDA software, but I'm not able to access the fluorescence chromatograms afterwards. The...

03 June 2014 5,804 1 View

SARS-CoV-2 blocked by Clinically Proven Protease Inhibitor

Hi, That looks very promising and soon we can get back to business as usual !? I recently found a publication online by Hoffmann et al. which appeared on March 5, 2020 in the journal Cell :...

01 January 1970 5,872 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

In a random graph, the number of paths that cannot be extended in an hamiltonian path, can be roughly be separated in two equal parts ?

In a random graph, the number of paths that cannot be further extended in an hamiltonian path or an hamiltonian cycle, can be roughly be separated in two equal parts? I have an idea for solve for...

07 January 2021 9,782 2 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View